Lecture No.137

Title: Nano-Engineered Biomaterials Prevent Device-Associated Infection Through Immunomodulation


Speaker: Bingyun Li, Associate Professor, Department of Orthopaedics, School of Medicine, West Virginia University


Date/Time: 2014-05-08,15:00


Venue: Room.308, Material Building A


Inviter: Prof.Weijie LV






Biomaterial-associated infection is one of the most common and problematic complications faced by millions of patients worldwide. The current antibiotic therapy strategies face challenges, the most serious of which are antibiotic resistance and intracellular persistence. Studies have shown that the systemic level of interleukin 12p70 (IL-12) decreases following major injuries resulting in decreased cell-mediated immune response. IL-12, a natural cytokine, plays a central role in cell-mediated immune response and bridges innate and adaptive immunities. In this presentation, IL-12 nanocoatings and microcapsules were developed using layer-by-layer nano-assembly, and their efficacy in preventing biomaterial-associated infection was examined. We found that IL-12 nanocoatings and microcapsules were effective in reducing infection in an open femur fracture rat model and were advantageous compared to conventional treatments including percutaneous and systemic injections. Moreover, exogenous IL-12 had additive effects in prophylaxis of infection in combination with conventional treatment (i.e. antibiotic therapy). In addition, nanoparticles targeting intracellular pathogens and nanoencapsulation of platelet rich plasma were briefly discussed. Our approaches could be a revolutionary step toward preventing biomaterial-associated infections using nonantibiotic (e.g. immunomodulation) treatments.


1. Wang X, Ding B, Li B*. (2013). Biomimetic electrospun nanofibrous structures for tissue engineering. Materials Today (Impact Factor 6.071), 16(6):229-41.

2. Li H, Hamza T, Tidwell JE, Clovis N, Li B*. (2013). Unique antimicrobial effects of platelet-rich plasma and its efficacy as a prophylaxis to prevent implant-associated spinal infection. Advanced Healthcare Materials. 2:1277-84.

3. Boyce BM, Lindsey BA, Clovis NB, Smith ES, Hobbs GR, Hubbard DF, Emery SE, Barnett JB, Li B*. (2012). Additive effects of exogenous IL-12 supplementation and antibiotic treatment in infection prophylaxis. J Orthop Res (Impact Factor 3.112) 30(2):196-202.

4. Zhao Q, Li H, Li B*. (2011). Nanoencapsulating living biological cells using electrostatic layer-by-layer self-assembly: platelets as a model. J Mater Res (Impact Factor 1.667) 26(2);347-351.

5.Jiang B, DeFusco E, Li B*. (2010). Polypeptide multilayer film co-delivers oppositely-charged drug molecules in sustained manners. Biomacromolecules (Impact Factor 4.502) 11(12);36307.

6. Li B*, Jiang B, Dietz MJ, Smith ES, Clovis NB, Rao KMK. (2010). Evaluation of local MCP-1 and IL-12 nanocoatings for infection prevention in open fractures. J Orthop Res (Impact Factor 3.112) 28;48-54.

7.Li B*, Jiang B, Boyce B, Lindsey B. (2009). Multilayer polypeptide nanoscale coatings incorporating IL-12 for the prevention of biomedical device-associated infections. Biomaterials (Impact Factor 7.365) 30;2552-8.

Location:Room.308, Material Building A